Our microscopy and digital imaging facility is equipped with a wide range of state-of-the art confocal microscopes, a transmission electron microscope, dissecting microscopes, and fluorescent microscopes.
Confocal Microscopes
The confocal microscope is a powerful tool for generating high-resolution images and 3D reconstructions of specimens. Confocal microscopy is superior to conventional fluorescent microscopy because of the clarity and depth of field that allow detailed investigation of the localization and function of various molecular components within a cell.
Our confocal microscopes include:
- An Olympus FV1000, multi-photon confocal microscope
- A Leica TCS NT confocal microscope
Our Olympus FV1000-MPE multi-photon confocal microscope features the following:
Spectral Scanner System with Spectrometer
Lasers
Spectra Physics Mai-Tai DeepSee Red/IR laser. Tunable 690-1020 nm
3-line Argon Laser (458/488/515 nm)
Combinational Red Laser Diode (LD559/635 nm)
Violet Laser Diode (LD405 nm)
Detectors
Dual Scan head for internal detectors: 2 spectral detectors, 2 standard detectors
Two channel external detectors
7215D-PSAC; InfraRed Viewer for Laser Alignment
Scan Optics with motorized Fluorescence Cube Turret
2nm high-resolution spectral scanning
Olympus Inverted Microscope
Olympus IX81 Inverted Microscope set
Motorized XYZ stage
Motorized DIC/Phase Condenser
Transmitted light detector
Mercury fluorescence detector
Olympus Objectives
PLAN APO |
1.25x |
0.04NA |
WD 5.1mm |
Air |
UPLANSAPO |
4x |
0.16NA |
WD 13mm |
Air |
UPLANSAPO |
10x |
0.4NA |
WD 3.1mm |
Air |
UMPLFL |
20x |
0.95NA |
WD 2mm |
Air |
XLPL |
25x |
1.05NA |
WD 2mm |
Air |
LUMPLFL |
40x |
0.8NA |
WD 3.3mm |
Water |
PLANAPO |
60x |
1.2NA |
WD 0.28 |
Water |
UPLSAPO |
100x |
1.4NA |
WD 0.17 |
Oil |
FV1000 MPE system: Optic specifications
| Lasers | Excitation wavelength |
Dyes & Fluorophores | Primary dichroic mirror (nm) | Secondary dichroic mirror (nm) |
| 405 LD | 405nm | DAPI, Hoechst | 405/488/559 | 490 |
| Multi-line Argon |
458nm | CFP | 458/515 | 510 |
| 488nm | Alexa 488, Oregon Green, FITC, GFP, EGFP, DiO, Cy2 |
405/488/559 | 560 | |
| 515nm | YFP, EYFP | 458/515 | 560 | |
| 559 LD | 559nm | Cy3, TRITC, DsRed, mCherry, Alexa 543, Alexa 594 |
405/488/559 | 575-620 640 |
| 635 LD | 633 nm | Alexa 633, Alexa 647, Alexa 688, Cy5, TO-PRO3 |
405/488/559/635 | 655-755 |
Incubation Chamber Unit for Live Cell Image
Tokai Hit INU/Stage-Top Incubator for Motorized Stage,
35mm Dish attachment with built-in digital gas mixer for supplying 5%CO2 + 95% O2 into the Chamber unit
Other Microscopes and Instruments
Transmission Electron Microscope (TEM) (Philips CM10)
Laser Capture Microdissection System (AutoPix LCM) (Arcturus LCM1110)
Zeiss inverted FluorescentScope (Axiovert 10)
Zeiss photomicroscope (Axiophot)
Olympus BX51 Microscope System (BX51 TRF)
Olympus SZ-40 Dissecting microscope
Olympus BH2 dual-head microscope
Olympus BH2 single-head microscope
Olympus binocular microscope (BX-41TF)
Micromaster binocular microscope
